Construct: shRNA TRCN0000122428
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_172071.1-4401s1c1
- DNA Barcode:
- GCTGACATCATAGGATGCTAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RC3H1 (149041)
- Gene Description:
- ring finger and CCCH-type domains 1
- Transcript:
- RefSeq NM_172071.1 (NON-CURRENT)
- Match location:
- Position 4401 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000122428)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTGACATCATAGGATGCTAA
- Hairpin Sequence:
-
5'-CCGG-GCTGACATCATAGGATGCTAA-CTCGAG-TTAGCATCCTATGATGTCAGC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTGACATCATAGGATGCTAACTCGAGTTAGCATCCTATGATGTCAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTGACATCATAGGATGCTAACTCGAGTTAGCATCCTATGATGTCAGC-3'
Other clones with same target sequence:
(none)
