Construct: shRNA TRCN0000122447

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173589.2-2818s1c1
DNA Barcode:
GCTCACAAATTCACGTGGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C11orf47 (283294)
Gene Description:
chromosome 11 open reading frame 47
Transcript:
RefSeq NM_173589.2 (NON-CURRENT)
Match location:
Position 2818 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000122447)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCACAAATTCACGTGGTTA
Hairpin Sequence:
5'-CCGG-GCTCACAAATTCACGTGGTTA-CTCGAG-TAACCACGTGAATTTGTGAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCACAAATTCACGTGGTTACTCGAGTAACCACGTGAATTTGTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCACAAATTCACGTGGTTACTCGAGTAACCACGTGAATTTGTGAGC-3'

Other clones with same target sequence:

(none)