Construct: shRNA TRCN0000122448

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177965.2-536s1c1
DNA Barcode:
GAAGAAAGGAACACGGGCATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C8orf37 (157657)
Gene Description:
chromosome 8 open reading frame 37
Transcript:
RefSeq NM_177965.2 (NON-CURRENT)
Match location:
Position 536 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000122448)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGAAAGGAACACGGGCATA
Hairpin Sequence:
5'-CCGG-GAAGAAAGGAACACGGGCATA-CTCGAG-TATGCCCGTGTTCCTTTCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGAAAGGAACACGGGCATACTCGAGTATGCCCGTGTTCCTTTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGAAAGGAACACGGGCATACTCGAGTATGCCCGTGTTCCTTTCTTC-3'

Other clones with same target sequence:

(none)