Construct: shRNA TRCN0000122462

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032589.2-365s1c1
DNA Barcode:
GCTGTTCATGCCAAGACCGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DSCR8 (84677)
Gene Description:
Down syndrome critical region 8
Transcript:
RefSeq NM_032589.2 (NON-CURRENT)
Match location:
Position 365 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000122462)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTTCATGCCAAGACCGAA
Hairpin Sequence:
5'-CCGG-GCTGTTCATGCCAAGACCGAA-CTCGAG-TTCGGTCTTGGCATGAACAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTTCATGCCAAGACCGAACTCGAGTTCGGTCTTGGCATGAACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTTCATGCCAAGACCGAACTCGAGTTCGGTCTTGGCATGAACAGC-3'

Other clones with same target sequence:

(none)