Construct: shRNA TRCN0000122472

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182616.1-292s1c1
DNA Barcode:
GTCACATCCATCGCCGTAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARPIN (348110)
Gene Description:
actin related protein 2/3 complex inhibitor
Transcript:
RefSeq NM_182616.1 (NON-CURRENT)
Match location:
Position 292 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000122472)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCACATCCATCGCCGTAAAT
Hairpin Sequence:
5'-CCGG-GTCACATCCATCGCCGTAAAT-CTCGAG-ATTTACGGCGATGGATGTGAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCACATCCATCGCCGTAAATCTCGAGATTTACGGCGATGGATGTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCACATCCATCGCCGTAAATCTCGAGATTTACGGCGATGGATGTGAC-3'

Other clones with same target sequence:

(none)