Construct: shRNA TRCN0000122479

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014453.2-561s1c1
DNA Barcode:
CATGGATATGAAGGAGGAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHMP2A (27243)
Gene Description:
charged multivesicular body protein 2A
Transcript:
RefSeq NM_014453.2 (NON-CURRENT)
Match location:
Position 561 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000122479)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGGATATGAAGGAGGAGAT
Hairpin Sequence:
5'-CCGG-CATGGATATGAAGGAGGAGAT-CTCGAG-ATCTCCTCCTTCATATCCATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGGATATGAAGGAGGAGATCTCGAGATCTCCTCCTTCATATCCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGGATATGAAGGAGGAGATCTCGAGATCTCCTCCTTCATATCCATG-3'

Other clones with same target sequence:

(none)