Construct: shRNA TRCN0000122494

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182552.2-2307s1c1
DNA Barcode:
CCTGTCCATCAAATCTGCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WDR27 (253769)
Gene Description:
WD repeat domain 27
Transcript:
RefSeq NM_182552.2 (NON-CURRENT)
Match location:
Position 2307 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000122494)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTCCATCAAATCTGCCAA
Hairpin Sequence:
5'-CCGG-CCTGTCCATCAAATCTGCCAA-CTCGAG-TTGGCAGATTTGATGGACAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTCCATCAAATCTGCCAACTCGAGTTGGCAGATTTGATGGACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTCCATCAAATCTGCCAACTCGAGTTGGCAGATTTGATGGACAGG-3'

Other clones with same target sequence:

(none)