Construct: shRNA TRCN0000122537

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007211.2-2150s1c1
DNA Barcode:
CCTGTTAGGTTACATCTGCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RASSF8 (11228)
Gene Description:
Ras association domain family member 8
Transcript:
RefSeq NM_007211.2 (NON-CURRENT)
Match location:
Position 2150 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000122537)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTTAGGTTACATCTGCTA
Hairpin Sequence:
5'-CCGG-CCTGTTAGGTTACATCTGCTA-CTCGAG-TAGCAGATGTAACCTAACAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTTAGGTTACATCTGCTACTCGAGTAGCAGATGTAACCTAACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTTAGGTTACATCTGCTACTCGAGTAGCAGATGTAACCTAACAGG-3'

Other clones with same target sequence:

(none)