Construct: shRNA TRCN0000122571

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012295.2-6929s1c1
DNA Barcode:
CCTCATGGCATCCTCCCTGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CABIN1 (23523)
Gene Description:
calcineurin binding protein 1
Transcript:
RefSeq NM_012295.2 (NON-CURRENT)
Match location:
Position 6929 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000122571)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCATGGCATCCTCCCTGTA
Hairpin Sequence:
5'-CCGG-CCTCATGGCATCCTCCCTGTA-CTCGAG-TACAGGGAGGATGCCATGAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCATGGCATCCTCCCTGTACTCGAGTACAGGGAGGATGCCATGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCATGGCATCCTCCCTGTACTCGAGTACAGGGAGGATGCCATGAGG-3'

Other clones with same target sequence:

(none)