Construct: shRNA TRCN0000122614

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139170.1-1219s1c1
DNA Barcode:
GCAACTTGAAGCGTGGGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C16orf71 (146562)
Gene Description:
chromosome 16 open reading frame 71
Transcript:
RefSeq NM_139170.1 (NON-CURRENT)
Match location:
Position 1219 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000122614)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAACTTGAAGCGTGGGATTT
Hairpin Sequence:
5'-CCGG-GCAACTTGAAGCGTGGGATTT-CTCGAG-AAATCCCACGCTTCAAGTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAACTTGAAGCGTGGGATTTCTCGAGAAATCCCACGCTTCAAGTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAACTTGAAGCGTGGGATTTCTCGAGAAATCCCACGCTTCAAGTTGC-3'

Other clones with same target sequence:

(none)