Construct: shRNA TRCN0000122819

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024781.1-1565s1c1
DNA Barcode:
GCTGTAAGCATCGCTGAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCDC102B (79839)
Gene Description:
coiled-coil domain containing 102B
Transcript:
RefSeq NM_024781.1 (NON-CURRENT)
Match location:
Position 1565 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000122819)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTAAGCATCGCTGAGAAA
Hairpin Sequence:
5'-CCGG-GCTGTAAGCATCGCTGAGAAA-CTCGAG-TTTCTCAGCGATGCTTACAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTAAGCATCGCTGAGAAACTCGAGTTTCTCAGCGATGCTTACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTAAGCATCGCTGAGAAACTCGAGTTTCTCAGCGATGCTTACAGC-3'

Other clones with same target sequence:

(none)