Construct: shRNA TRCN0000122876

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_375080.1-232s1c1
DNA Barcode:
GCAAATCTTCGACGATCCCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATG14 (22863)
Gene Description:
autophagy related 14
Transcript:
RefSeq XM_375080.1 (NON-CURRENT)
Match location:
Position 232 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000122876)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAATCTTCGACGATCCCAT
Hairpin Sequence:
5'-CCGG-GCAAATCTTCGACGATCCCAT-CTCGAG-ATGGGATCGTCGAAGATTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAATCTTCGACGATCCCATCTCGAGATGGGATCGTCGAAGATTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAATCTTCGACGATCCCATCTCGAGATGGGATCGTCGAAGATTTGC-3'

Other clones with same target sequence:

(none)