Construct: shRNA TRCN0000122916

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003581.2-788s1c1
DNA Barcode:
GCCTTCGTCAAGTTCGCCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NCK2 (8440)
Gene Description:
NCK adaptor protein 2
Transcript:
RefSeq NM_003581.2 (NON-CURRENT)
Match location:
Position 788 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000122916)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTCGTCAAGTTCGCCTAT
Hairpin Sequence:
5'-CCGG-GCCTTCGTCAAGTTCGCCTAT-CTCGAG-ATAGGCGAACTTGACGAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTCGTCAAGTTCGCCTATCTCGAGATAGGCGAACTTGACGAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTCGTCAAGTTCGCCTATCTCGAGATAGGCGAACTTGACGAAGGC-3'

Other clones with same target sequence:

(none)