Construct: shRNA TRCN0000122928

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033375.3-1952s1c1
DNA Barcode:
CCCATTATGAGCCAGTGCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYO1C (4641)
Gene Description:
myosin IC
Transcript:
RefSeq NM_033375.3 (NON-CURRENT)
Match location:
Position 1952 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000122928)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATTATGAGCCAGTGCTTT
Hairpin Sequence:
5'-CCGG-CCCATTATGAGCCAGTGCTTT-CTCGAG-AAAGCACTGGCTCATAATGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATTATGAGCCAGTGCTTTCTCGAGAAAGCACTGGCTCATAATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATTATGAGCCAGTGCTTTCTCGAGAAAGCACTGGCTCATAATGGG-3'

Other clones with same target sequence:

(none)