Construct: shRNA TRCN0000122938

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002456.4-474s1c1
DNA Barcode:
GACACAGTTCAATCAGTATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MUC1 (4582)
Gene Description:
mucin 1, cell surface associated
Transcript:
RefSeq NM_002456.4 (NON-CURRENT)
Match location:
Position 474 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000122938)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACACAGTTCAATCAGTATAA
Hairpin Sequence:
5'-CCGG-GACACAGTTCAATCAGTATAA-CTCGAG-TTATACTGATTGAACTGTGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACACAGTTCAATCAGTATAACTCGAGTTATACTGATTGAACTGTGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACACAGTTCAATCAGTATAACTCGAGTTATACTGATTGAACTGTGTC-3'

Other clones with same target sequence:

(none)