Construct: shRNA TRCN0000122963

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013327.3-985s1c1
DNA Barcode:
CTTCGATCAGAAGGTCCACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PARVB (29780)
Gene Description:
parvin beta
Transcript:
RefSeq NM_013327.3 (NON-CURRENT)
Match location:
Position 985 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000122963)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCGATCAGAAGGTCCACAA
Hairpin Sequence:
5'-CCGG-CTTCGATCAGAAGGTCCACAA-CTCGAG-TTGTGGACCTTCTGATCGAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCGATCAGAAGGTCCACAACTCGAGTTGTGGACCTTCTGATCGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCGATCAGAAGGTCCACAACTCGAGTTGTGGACCTTCTGATCGAAG-3'

Other clones with same target sequence:

  1. TRCN0000343680