Construct: shRNA TRCN0000122965

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020812.1-5464s1c1
DNA Barcode:
GCCTACATCCAGATCACGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DOCK6 (57572)
Gene Description:
dedicator of cytokinesis 6
Transcript:
RefSeq NM_020812.1 (NON-CURRENT)
Match location:
Position 5464 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000122965)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTACATCCAGATCACGTAT
Hairpin Sequence:
5'-CCGG-GCCTACATCCAGATCACGTAT-CTCGAG-ATACGTGATCTGGATGTAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTACATCCAGATCACGTATCTCGAGATACGTGATCTGGATGTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTACATCCAGATCACGTATCTCGAGATACGTGATCTGGATGTAGGC-3'

Other clones with same target sequence:

(none)