Construct: shRNA TRCN0000122994

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003931.2-3073s1c1
DNA Barcode:
CCCACTTATATATTGTGTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WASF1 (8936)
Gene Description:
WASP family member 1
Transcript:
RefSeq NM_003931.2 (NON-CURRENT)
Match location:
Position 3073 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000122994)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCACTTATATATTGTGTGAT
Hairpin Sequence:
5'-CCGG-CCCACTTATATATTGTGTGAT-CTCGAG-ATCACACAATATATAAGTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCACTTATATATTGTGTGATCTCGAGATCACACAATATATAAGTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCACTTATATATTGTGTGATCTCGAGATCACACAATATATAAGTGGG-3'

Other clones with same target sequence:

(none)