Construct: shRNA TRCN0000123004

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020066.3-6122s1c1
DNA Barcode:
GCTCTCCATAACTATGTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FMN2 (56776)
Gene Description:
formin 2
Transcript:
RefSeq NM_020066.3 (NON-CURRENT)
Match location:
Position 6122 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123004)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTCCATAACTATGTATTT
Hairpin Sequence:
5'-CCGG-GCTCTCCATAACTATGTATTT-CTCGAG-AAATACATAGTTATGGAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTCCATAACTATGTATTTCTCGAGAAATACATAGTTATGGAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTCCATAACTATGTATTTCTCGAGAAATACATAGTTATGGAGAGC-3'

Other clones with same target sequence:

(none)