Construct: shRNA TRCN0000123011

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032714.1-521s1c1
DNA Barcode:
CCTGGACACATCCAACGTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
INF2 (64423)
Gene Description:
inverted formin, FH2 and WH2 domain containing
Transcript:
RefSeq NM_032714.1 (NON-CURRENT)
Match location:
Position 521 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123011)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGACACATCCAACGTGAT
Hairpin Sequence:
5'-CCGG-CCTGGACACATCCAACGTGAT-CTCGAG-ATCACGTTGGATGTGTCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGACACATCCAACGTGATCTCGAGATCACGTTGGATGTGTCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGACACATCCAACGTGATCTCGAGATCACGTTGGATGTGTCCAGG-3'

Other clones with same target sequence:

(none)