Construct: shRNA TRCN0000123014

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144658.2-6302s1c1
DNA Barcode:
CGTGACAATGAGACTGACCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DOCK11 (139818)
Gene Description:
dedicator of cytokinesis 11
Transcript:
RefSeq NM_144658.2 (NON-CURRENT)
Match location:
Position 6302 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123014)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGACAATGAGACTGACCTT
Hairpin Sequence:
5'-CCGG-CGTGACAATGAGACTGACCTT-CTCGAG-AAGGTCAGTCTCATTGTCACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGACAATGAGACTGACCTTCTCGAGAAGGTCAGTCTCATTGTCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGACAATGAGACTGACCTTCTCGAGAAGGTCAGTCTCATTGTCACG-3'

Other clones with same target sequence:

(none)