Construct: shRNA TRCN0000123016

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144658.2-2411s1c1
DNA Barcode:
CCAGGCTACTTGAATCTGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DOCK11 (139818)
Gene Description:
dedicator of cytokinesis 11
Transcript:
RefSeq NM_144658.2 (NON-CURRENT)
Match location:
Position 2411 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123016)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGCTACTTGAATCTGAAT
Hairpin Sequence:
5'-CCGG-CCAGGCTACTTGAATCTGAAT-CTCGAG-ATTCAGATTCAAGTAGCCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGCTACTTGAATCTGAATCTCGAGATTCAGATTCAAGTAGCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGCTACTTGAATCTGAATCTCGAGATTCAGATTCAAGTAGCCTGG-3'

Other clones with same target sequence:

(none)