Construct: shRNA TRCN0000123035

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012287.3-2334s1c1
DNA Barcode:
GCTGATATAGTCACCTTGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACAP2 (23527)
Gene Description:
ArfGAP with coiled-coil, ankyrin repeat and PH domains 2
Transcript:
RefSeq NM_012287.3 (NON-CURRENT)
Match location:
Position 2334 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123035)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGATATAGTCACCTTGTTA
Hairpin Sequence:
5'-CCGG-GCTGATATAGTCACCTTGTTA-CTCGAG-TAACAAGGTGACTATATCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGATATAGTCACCTTGTTACTCGAGTAACAAGGTGACTATATCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGATATAGTCACCTTGTTACTCGAGTAACAAGGTGACTATATCAGC-3'

Other clones with same target sequence:

(none)