Construct: shRNA TRCN0000123040

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003088.2-1482s1c1
DNA Barcode:
CGACTATAACAAGGTGGCCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FSCN1 (6624)
Gene Description:
fascin actin-bundling protein 1
Transcript:
RefSeq NM_003088.2 (NON-CURRENT)
Match location:
Position 1482 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123040)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACTATAACAAGGTGGCCAT
Hairpin Sequence:
5'-CCGG-CGACTATAACAAGGTGGCCAT-CTCGAG-ATGGCCACCTTGTTATAGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACTATAACAAGGTGGCCATCTCGAGATGGCCACCTTGTTATAGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACTATAACAAGGTGGCCATCTCGAGATGGCCACCTTGTTATAGTCG-3'

Other clones with same target sequence:

  1. TRCN0000289052