Construct: shRNA TRCN0000123088

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012334.1-4075s1c1
DNA Barcode:
GATAGGACTTTCCACCTGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYO10 (4651)
Gene Description:
myosin X
Transcript:
RefSeq NM_012334.1 (NON-CURRENT)
Match location:
Position 4075 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123088)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATAGGACTTTCCACCTGATT
Hairpin Sequence:
5'-CCGG-GATAGGACTTTCCACCTGATT-CTCGAG-AATCAGGTGGAAAGTCCTATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATAGGACTTTCCACCTGATTCTCGAGAATCAGGTGGAAAGTCCTATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATAGGACTTTCCACCTGATTCTCGAGAATCAGGTGGAAAGTCCTATC-3'

Other clones with same target sequence:

  1. TRCN0000286557