Construct: shRNA TRCN0000123097

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005708.2-1890s1c1
DNA Barcode:
GCACAGCAAAGCCAGATACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GPC6 (10082)
Gene Description:
glypican 6
Transcript:
RefSeq NM_005708.2 (NON-CURRENT)
Match location:
Position 1890 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123097)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAGCAAAGCCAGATACTT
Hairpin Sequence:
5'-CCGG-GCACAGCAAAGCCAGATACTT-CTCGAG-AAGTATCTGGCTTTGCTGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAGCAAAGCCAGATACTTCTCGAGAAGTATCTGGCTTTGCTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAGCAAAGCCAGATACTTCTCGAGAAGTATCTGGCTTTGCTGTGC-3'

Other clones with same target sequence:

(none)