Construct: shRNA TRCN0000123099

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181334.3-1573s1c1
DNA Barcode:
CACTCTGTATATTTCGAGCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRR5-ARHGAP8 (553158)
Gene Description:
PRR5-ARHGAP8 readthrough
Transcript:
RefSeq NM_181334.3 (NON-CURRENT)
Match location:
Position 1573 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123099)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTCTGTATATTTCGAGCTA
Hairpin Sequence:
5'-CCGG-CACTCTGTATATTTCGAGCTA-CTCGAG-TAGCTCGAAATATACAGAGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTCTGTATATTTCGAGCTACTCGAGTAGCTCGAAATATACAGAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTCTGTATATTTCGAGCTACTCGAGTAGCTCGAAATATACAGAGTG-3'

Other clones with same target sequence:

(none)