Construct: shRNA TRCN0000123103

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181334.3-465s1c1
DNA Barcode:
CGATTATACCATCGTCTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRR5-ARHGAP8 (553158)
Gene Description:
PRR5-ARHGAP8 readthrough
Transcript:
RefSeq NM_181334.3 (NON-CURRENT)
Match location:
Position 465 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123103)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGATTATACCATCGTCTATTT
Hairpin Sequence:
5'-CCGG-CGATTATACCATCGTCTATTT-CTCGAG-AAATAGACGATGGTATAATCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGATTATACCATCGTCTATTTCTCGAGAAATAGACGATGGTATAATCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGATTATACCATCGTCTATTTCTCGAGAAATAGACGATGGTATAATCG-3'

Other clones with same target sequence:

(none)