Construct: shRNA TRCN0000123230

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006932.3-1956s1c1
DNA Barcode:
GCTGAGGAGCTGATGACTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMTN (6525)
Gene Description:
smoothelin
Transcript:
RefSeq NM_006932.3 (NON-CURRENT)
Match location:
Position 1956 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123230)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAGGAGCTGATGACTATT
Hairpin Sequence:
5'-CCGG-GCTGAGGAGCTGATGACTATT-CTCGAG-AATAGTCATCAGCTCCTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAGGAGCTGATGACTATTCTCGAGAATAGTCATCAGCTCCTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAGGAGCTGATGACTATTCTCGAGAATAGTCATCAGCTCCTCAGC-3'

Other clones with same target sequence:

(none)