Construct: shRNA TRCN0000123252

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022068.1-1392s1c1
DNA Barcode:
GCTATGGTATTATGGGATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIEZO2 (63895)
Gene Description:
piezo type mechanosensitive ion channel component 2
Transcript:
RefSeq NM_022068.1 (NON-CURRENT)
Match location:
Position 1392 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123252)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTATGGTATTATGGGATTAT
Hairpin Sequence:
5'-CCGG-GCTATGGTATTATGGGATTAT-CTCGAG-ATAATCCCATAATACCATAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTATGGTATTATGGGATTATCTCGAGATAATCCCATAATACCATAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTATGGTATTATGGGATTATCTCGAGATAATCCCATAATACCATAGC-3'

Other clones with same target sequence:

(none)