Construct: shRNA TRCN0000123262

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014178.6-1129s1c1
DNA Barcode:
GCCAGAGTTTATTAACTGCCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STXBP6 (29091)
Gene Description:
syntaxin binding protein 6
Transcript:
RefSeq NM_014178.6 (NON-CURRENT)
Match location:
Position 1129 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123262)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGAGTTTATTAACTGCCA
Hairpin Sequence:
5'-CCGG-GCCAGAGTTTATTAACTGCCA-CTCGAG-TGGCAGTTAATAAACTCTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGAGTTTATTAACTGCCACTCGAGTGGCAGTTAATAAACTCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGAGTTTATTAACTGCCACTCGAGTGGCAGTTAATAAACTCTGGC-3'

Other clones with same target sequence:

(none)