Construct: shRNA TRCN0000123283

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018099.3-823s1c1
DNA Barcode:
CGATGCTATTATTGACGAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAR2 (55711)
Gene Description:
fatty acyl-CoA reductase 2
Transcript:
RefSeq NM_018099.3 (NON-CURRENT)
Match location:
Position 823 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123283)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGATGCTATTATTGACGAGAT
Hairpin Sequence:
5'-CCGG-CGATGCTATTATTGACGAGAT-CTCGAG-ATCTCGTCAATAATAGCATCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGATGCTATTATTGACGAGATCTCGAGATCTCGTCAATAATAGCATCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGATGCTATTATTGACGAGATCTCGAGATCTCGTCAATAATAGCATCG-3'

Other clones with same target sequence:

(none)