Construct: shRNA TRCN0000123328

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022121.2-582s1c1
DNA Barcode:
GCAGCCACGATTATCCTGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PERP (64065)
Gene Description:
p53 apoptosis effector related to PMP22
Transcript:
RefSeq NM_022121.2 (NON-CURRENT)
Match location:
Position 582 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123328)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCCACGATTATCCTGATT
Hairpin Sequence:
5'-CCGG-GCAGCCACGATTATCCTGATT-CTCGAG-AATCAGGATAATCGTGGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCCACGATTATCCTGATTCTCGAGAATCAGGATAATCGTGGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCCACGATTATCCTGATTCTCGAGAATCAGGATAATCGTGGCTGC-3'

Other clones with same target sequence:

(none)