Construct: shRNA TRCN0000123343

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022484.3-1926s1c1
DNA Barcode:
GCCTTGACACACTTATAGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMEM168 (64418)
Gene Description:
transmembrane protein 168
Transcript:
RefSeq NM_022484.3 (NON-CURRENT)
Match location:
Position 1926 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123343)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTGACACACTTATAGAAT
Hairpin Sequence:
5'-CCGG-GCCTTGACACACTTATAGAAT-CTCGAG-ATTCTATAAGTGTGTCAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTGACACACTTATAGAATCTCGAGATTCTATAAGTGTGTCAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTGACACACTTATAGAATCTCGAGATTCTATAAGTGTGTCAAGGC-3'

Other clones with same target sequence:

(none)