Construct: shRNA TRCN0000123364

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027188.2-2248s1c1
DNA Barcode:
GCTGAGTAATAGCCTGTTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smyd3 (69726)
Gene Description:
SET and MYND domain containing 3
Transcript:
RefSeq NM_027188.2 (NON-CURRENT)
Match location:
Position 2248 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123364)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAGTAATAGCCTGTTATT
Hairpin Sequence:
5'-CCGG-GCTGAGTAATAGCCTGTTATT-CTCGAG-AATAACAGGCTATTACTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAGTAATAGCCTGTTATTCTCGAGAATAACAGGCTATTACTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAGTAATAGCCTGTTATTCTCGAGAATAACAGGCTATTACTCAGC-3'

Other clones with same target sequence:

(none)