Construct: shRNA TRCN0000123457

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145139.2-160s1c1
DNA Barcode:
GCAGCAGACATATCAGGTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eif3l (223691)
Gene Description:
eukaryotic translation initiation factor 3, subunit L
Transcript:
RefSeq NM_145139.2 (CURRENT)
Match location:
Position 160 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123457)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCAGACATATCAGGTGAT
Hairpin Sequence:
5'-CCGG-GCAGCAGACATATCAGGTGAT-CTCGAG-ATCACCTGATATGTCTGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCAGACATATCAGGTGATCTCGAGATCACCTGATATGTCTGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCAGACATATCAGGTGATCTCGAGATCACCTGATATGTCTGCTGC-3'

Other clones with same target sequence:

  1. TRCN0000317011