Construct: shRNA TRCN0000123468

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144948.2-633s1c1
DNA Barcode:
GCACTGTCATCACAGCGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rbm7 (67010)
Gene Description:
RNA binding motif protein 7
Transcript:
RefSeq NM_144948.2 (NON-CURRENT)
Match location:
Position 633 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123468)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTGTCATCACAGCGTAAA
Hairpin Sequence:
5'-CCGG-GCACTGTCATCACAGCGTAAA-CTCGAG-TTTACGCTGTGATGACAGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTGTCATCACAGCGTAAACTCGAGTTTACGCTGTGATGACAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTGTCATCACAGCGTAAACTCGAGTTTACGCTGTGATGACAGTGC-3'

Other clones with same target sequence:

(none)