Construct: shRNA TRCN0000123479

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144922.1-2724s1c1
DNA Barcode:
GCTTCTAATAGCACCTGCCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hnrnpul1 (232989)
Gene Description:
heterogeneous nuclear ribonucleoprotein U-like 1
Transcript:
RefSeq NM_144922.1 (NON-CURRENT)
Match location:
Position 2724 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123479)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCTAATAGCACCTGCCTT
Hairpin Sequence:
5'-CCGG-GCTTCTAATAGCACCTGCCTT-CTCGAG-AAGGCAGGTGCTATTAGAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCTAATAGCACCTGCCTTCTCGAGAAGGCAGGTGCTATTAGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCTAATAGCACCTGCCTTCTCGAGAAGGCAGGTGCTATTAGAAGC-3'

Other clones with same target sequence:

  1. TRCN0000317907