Construct: shRNA TRCN0000123515

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139065.1-1818s1c1
DNA Barcode:
CCGTCCAATAACTCCTGTGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rbm47 (245945)
Gene Description:
RNA binding motif protein 47
Transcript:
RefSeq NM_139065.1 (NON-CURRENT)
Match location:
Position 1818 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123515)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTCCAATAACTCCTGTGTA
Hairpin Sequence:
5'-CCGG-CCGTCCAATAACTCCTGTGTA-CTCGAG-TACACAGGAGTTATTGGACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTCCAATAACTCCTGTGTACTCGAGTACACAGGAGTTATTGGACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTCCAATAACTCCTGTGTACTCGAGTACACAGGAGTTATTGGACGG-3'

Other clones with same target sequence:

(none)