Construct: shRNA TRCN0000123515
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_139065.1-1818s1c1
- DNA Barcode:
- CCGTCCAATAACTCCTGTGTA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Rbm47 (245945)
- Gene Description:
- RNA binding motif protein 47
- Transcript:
- RefSeq NM_139065.1 (NON-CURRENT)
- Match location:
- Position 1818 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000123515)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCGTCCAATAACTCCTGTGTA
- Hairpin Sequence:
-
5'-CCGG-CCGTCCAATAACTCCTGTGTA-CTCGAG-TACACAGGAGTTATTGGACGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCGTCCAATAACTCCTGTGTACTCGAGTACACAGGAGTTATTGGACGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCGTCCAATAACTCCTGTGTACTCGAGTACACAGGAGTTATTGGACGG-3'
Other clones with same target sequence:
(none)
