Construct: shRNA TRCN0000123554

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152946.1-1893s1c1
DNA Barcode:
GCTACAGTCGAGAGTACAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Clasrp (53609)
Gene Description:
CLK4-associating serine/arginine rich protein
Transcript:
RefSeq NM_152946.1 (NON-CURRENT)
Match location:
Position 1893 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123554)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTACAGTCGAGAGTACAGTT
Hairpin Sequence:
5'-CCGG-GCTACAGTCGAGAGTACAGTT-CTCGAG-AACTGTACTCTCGACTGTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTACAGTCGAGAGTACAGTTCTCGAGAACTGTACTCTCGACTGTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTACAGTCGAGAGTACAGTTCTCGAGAACTGTACTCTCGACTGTAGC-3'

Other clones with same target sequence:

(none)