Construct: shRNA TRCN0000123599

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025845.1-2649s1c1
DNA Barcode:
GCCCTATACTAAGGAGTGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prpf38b (66921)
Gene Description:
PRP38 pre-mRNA processing factor 38 (yeast) domain containing B
Transcript:
RefSeq NM_025845.1 (NON-CURRENT)
Match location:
Position 2649 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123599)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTATACTAAGGAGTGATT
Hairpin Sequence:
5'-CCGG-GCCCTATACTAAGGAGTGATT-CTCGAG-AATCACTCCTTAGTATAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTATACTAAGGAGTGATTCTCGAGAATCACTCCTTAGTATAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTATACTAAGGAGTGATTCTCGAGAATCACTCCTTAGTATAGGGC-3'

Other clones with same target sequence:

(none)