Construct: shRNA TRCN0000123603

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025845.1-861s1c1
DNA Barcode:
CCATTGGAGAGATGCTTCGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prpf38b (66921)
Gene Description:
PRP38 pre-mRNA processing factor 38 (yeast) domain containing B
Transcript:
RefSeq NM_025845.1 (NON-CURRENT)
Match location:
Position 861 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123603)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATTGGAGAGATGCTTCGTT
Hairpin Sequence:
5'-CCGG-CCATTGGAGAGATGCTTCGTT-CTCGAG-AACGAAGCATCTCTCCAATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATTGGAGAGATGCTTCGTTCTCGAGAACGAAGCATCTCTCCAATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATTGGAGAGATGCTTCGTTCTCGAGAACGAAGCATCTCTCCAATGG-3'

Other clones with same target sequence:

(none)