Construct: shRNA TRCN0000123622

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022408.1-619s1c1
DNA Barcode:
GAGAAGCGACAGAAAGATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dgcr14 (27886)
Gene Description:
DiGeorge syndrome critical region gene 14
Transcript:
RefSeq NM_022408.1 (NON-CURRENT)
Match location:
Position 619 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123622)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAAGCGACAGAAAGATAAT
Hairpin Sequence:
5'-CCGG-GAGAAGCGACAGAAAGATAAT-CTCGAG-ATTATCTTTCTGTCGCTTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAAGCGACAGAAAGATAATCTCGAGATTATCTTTCTGTCGCTTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAAGCGACAGAAAGATAATCTCGAGATTATCTTTCTGTCGCTTCTC-3'

Other clones with same target sequence:

  1. TRCN0000324243