Construct: shRNA TRCN0000123671

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133934.2-460s1c1
DNA Barcode:
CCCAGGAAATCGAGGTTACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Isy1 (57905)
Gene Description:
ISY1 splicing factor homolog
Transcript:
RefSeq NM_133934.2 (NON-CURRENT)
Match location:
Position 460 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123671)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGGAAATCGAGGTTACAA
Hairpin Sequence:
5'-CCGG-CCCAGGAAATCGAGGTTACAA-CTCGAG-TTGTAACCTCGATTTCCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGGAAATCGAGGTTACAACTCGAGTTGTAACCTCGATTTCCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGGAAATCGAGGTTACAACTCGAGTTGTAACCTCGATTTCCTGGG-3'

Other clones with same target sequence:

(none)