Construct: shRNA TRCN0000123684

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025299.1-1031s1c1
DNA Barcode:
CCCTACTTACAAACACCAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Txnl4a (27366)
Gene Description:
thioredoxin-like 4A
Transcript:
RefSeq NM_025299.1 (NON-CURRENT)
Match location:
Position 1031 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123684)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTACTTACAAACACCAGTT
Hairpin Sequence:
5'-CCGG-CCCTACTTACAAACACCAGTT-CTCGAG-AACTGGTGTTTGTAAGTAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTACTTACAAACACCAGTTCTCGAGAACTGGTGTTTGTAAGTAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTACTTACAAACACCAGTTCTCGAGAACTGGTGTTTGTAAGTAGGG-3'

Other clones with same target sequence:

(none)