Construct: shRNA TRCN0000123691

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028597.1-637s1c1
DNA Barcode:
CCTCAGCTACCCAGAATTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Thoc3 (73666)
Gene Description:
THO complex 3
Transcript:
RefSeq NM_028597.1 (NON-CURRENT)
Match location:
Position 637 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123691)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCAGCTACCCAGAATTGAA
Hairpin Sequence:
5'-CCGG-CCTCAGCTACCCAGAATTGAA-CTCGAG-TTCAATTCTGGGTAGCTGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCAGCTACCCAGAATTGAACTCGAGTTCAATTCTGGGTAGCTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCAGCTACCCAGAATTGAACTCGAGTTCAATTCTGGGTAGCTGAGG-3'

Other clones with same target sequence:

  1. TRCN0000324319