Construct: shRNA TRCN0000123704

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029012.2-1895s1c1
DNA Barcode:
GCACATAGATATCTGTGAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sppl3 (74585)
Gene Description:
signal peptide peptidase 3
Transcript:
RefSeq NM_029012.2 (CURRENT)
Match location:
Position 1895 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123704)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACATAGATATCTGTGAGTA
Hairpin Sequence:
5'-CCGG-GCACATAGATATCTGTGAGTA-CTCGAG-TACTCACAGATATCTATGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACATAGATATCTGTGAGTACTCGAGTACTCACAGATATCTATGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACATAGATATCTGTGAGTACTCGAGTACTCACAGATATCTATGTGC-3'

Other clones with same target sequence:

  1. TRCN0000309641