Construct: shRNA TRCN0000123712

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053193.1-1155s1c1
DNA Barcode:
CCGAGCGTTTCACTTTGACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cpsf1 (94230)
Gene Description:
cleavage and polyadenylation specific factor 1
Transcript:
RefSeq NM_053193.1 (NON-CURRENT)
Match location:
Position 1155 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123712)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGCGTTTCACTTTGACAA
Hairpin Sequence:
5'-CCGG-CCGAGCGTTTCACTTTGACAA-CTCGAG-TTGTCAAAGTGAAACGCTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGCGTTTCACTTTGACAACTCGAGTTGTCAAAGTGAAACGCTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGCGTTTCACTTTGACAACTCGAGTTGTCAAAGTGAAACGCTCGG-3'

Other clones with same target sequence:

(none)