Construct: shRNA TRCN0000123717

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010760.1-458s1c1
DNA Barcode:
GATTGGATTACACTTCAAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Magoh (17149)
Gene Description:
mago homolog, exon junction complex core component
Transcript:
RefSeq NM_010760.1 (NON-CURRENT)
Match location:
Position 458 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123717)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTGGATTACACTTCAAGAT
Hairpin Sequence:
5'-CCGG-GATTGGATTACACTTCAAGAT-CTCGAG-ATCTTGAAGTGTAATCCAATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTGGATTACACTTCAAGATCTCGAGATCTTGAAGTGTAATCCAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTGGATTACACTTCAAGATCTCGAGATCTTGAAGTGTAATCCAATC-3'

Other clones with same target sequence:

  1. TRCN0000317284