Construct: shRNA TRCN0000123737

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019733.1-566s1c1
DNA Barcode:
CGACTAGAGTTTGCTAAGGCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rbpms (19663)
Gene Description:
RNA binding protein gene with multiple splicing
Transcript:
RefSeq NM_019733.1 (NON-CURRENT)
Match location:
Position 566 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123737)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACTAGAGTTTGCTAAGGCA
Hairpin Sequence:
5'-CCGG-CGACTAGAGTTTGCTAAGGCA-CTCGAG-TGCCTTAGCAAACTCTAGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACTAGAGTTTGCTAAGGCACTCGAGTGCCTTAGCAAACTCTAGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACTAGAGTTTGCTAAGGCACTCGAGTGCCTTAGCAAACTCTAGTCG-3'

Other clones with same target sequence:

  1. TRCN0000164568